Guven Lab. on Therapeutic Bioengineering

OVERVIEW

Advances in stem cell biology and genetics with bioengineering science continuously lead breakthroughs and innovations in biotechnology. We harmonize fundamental sciences such as chemistry, biology, and physics with principles of engineering to generate innovative and effective therapeutic approaches, tissue mimicries and medical microdevices for medicine and pharmaceutical sciences. Our group focuses on bioengineering of novel native-like 3D cellular microenvironments, organ-on-a-chip platforms, and cell therapy approaches and integrates them for human health.

RESEARCH INTERESTS

Tissue and cellular microenvironment engineering: An understanding of how stem cells respond to physical and chemical stimulations from their extracellular environment is essential for utilizing the therapeutic potential of stem cells. We focus on developing novel biomaterials to construct cellular niches that can provide control over the stem cell differentiation and functionalization. We build tissues for regenerative medicine and pharmaceutical applications by designing and defining the cell-material and cell-cell interactions. Utilizing the bioengineering principles we develop bioreactor systems, tissue fabrication and assembly techniques for bottom-up and top-down tissue engineering.

Bio-mimicry organ-on-a-chip platform technologies for biomedical research: Organs-on-chips technologies provide a great platform for the investigation of fundamental mechanisms of organ physiology and disease. Such tools enable control over the physical and chemical factors of an in vitro organ models. Our group focuses on designing and engineering BioMEMS for basic research on cell physiology, in vitro disease models (e.g., neurodegenerative diseases, rare diseases, and cancer), diagnostics and pharmaceutical research.

Personalized and cellular therapies: Cell-based therapies provide unique and personalized approaches in current clinical treatments. Autologous and allogenic adult mesenchymal stem cells from bone marrow, adipose tissue, Wharton jelly and dental pulp, as well as limbal stem cells, and specialized cells (e.g., chondrocytes, hepatocytes) have been demonstrated to be effective in clinics. We develop novel therapeutic methods from bench to bedside by utilizing of cell-based therapies and intraoperative approaches in clinics.

RESEARCH HIGHLIGHTS

Biological organizations are highly hierarchical in architecture from the molecular to the macroscopic level. Such complexity in tissues is formed by building units that regulate and provide the system function. To mimic and bioengineer functional tissues, we need biomanufacturing tools that can generate and manipulate multi-scale building blocks. Bottom-up tissue engineering approaches focus on creating tissues by assembling heterogeneous building units such as biomacromolecules, cells, and cell-loaded microscale hydrogels in a multiscale manner. In our recent works, we demonstrated different cell and tissue assembly approaches utilizing acoustic, magnetic, micro robotic and bioprinting technologies to generate model platforms and tissues. Bioengineering approaches are essential for current therapeutic and diagnostic applications in medicine. Together with advances in genetics and cell biology, bioengineering tools provide key elements for novel and innovative medical technologies for human health.

Engineering platforms and systems for biofabrication of 3D tissues and microenvironments.

Representative bioengineered tissue constructs A. Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue (Guven et al. 2011), B. In vitro endothelial capillaries from stromal vascular fraction (Guven et al. 2011), C. Enhanced engraftment and functionality of bio-engineeredautologous dermo-epidermal skin grafts pre-vascularized with adipose-derived cells (Klar et al. 2014), D. Direct neural differentiation of mouse embryonic stem cells in 3D microenvironments, E. Biotunable acoustic node assembly of hepatic organoids (Chen et al. 2015), F. Guided and magnetic self-assembly of tunable magnetoceptive cardiac microtissues (Tasoglu et al. 2014).

Selected Publications

Utine CA, Ayhan Z, Durmaz Engin C. Effect of intracorneal ring segment implantation on corneal asphericity. Int J Ophthalmol. 2018 Aug ; 11 (8): 1303-1307. doi:10.18240/ijo.2018.08.09.

Utine CA, Durmaz Engin C, Ayhan Z. Effects of Preoperative Topometric Indices on Visual Gain After Intracorneal Ring Segment Implantation For Keratoconus. Eye & Contact Lens Science and Clinical Practice. 2018 Nov ; 44 (2): S387-S391. doi:10.1097/ICL.0000000000000490.

Raciha Beril Küçümen, Berna Şahan, Canan Aslı Yıldırım, Ferda Çiftçi. Evaluation of Corneal Biomechanical Changes After Collagen Crosslinking in Patients with Progressive Keratoconus by Ocular Response Analyzer. Turk J Ophthalmol. 2018 Aug ; 48 (4): 160-165. doi:10.4274/tjo.56750.

Utine CA, Stern M, Akpek EK. Immunopathological Features of Severe Chronic Atopic Keratoconjunctivitis and Effects of Topical Cyclosporine Treatment. Ocular immunology and inflammation. 2018 Sep ; 6 1-10. doi:10.1080/09273948.2018.1511811.

Çakir H, Utine CA. Management of Reverse Pellucid Type Topography. Eye & contact lens. 2018 Nov ; 44 (Suppl 2): S131-S136. doi:10.1097/ICL.0000000000000423.

Kocaoğlu G, Utine CA, Yaman A, Men S. Orbital Apex Syndrome Secondary to Herpes Zoster Ophthalmicus.. Turkish journal of ophthalmology. 2018 Feb ; 48 (1): 42-46. doi:10.4274/tjo.02256.

Arikan G, Karatas E, Lebe B, Ayhan Z, Utine CA, Kutsoylu OE, Gunenc U, Yilmaz O. Topically applied 1% voriconazole induces dysplastic changes on the ocular surface: animal study. Cutaneous and ocular toxicology. 2018 Dec ; 37 (4): 328-331. doi:10.1080/15569527.2018.1463237.

Utine CA, Engin Durmaz C, Koçak N. Corneal matrix repair therapy with the regenerating agent in neurotrophic persistent epithelial defects. International journal of ophthalmology. 2017 Dec ; 10 (12): 1935-1939. doi:10.18240/ijo.2017.12.25.

Mathews PM, Ramulu PY, Swenor BS, Utine CA, Rubin GS, Akpek EK. Functional impairment of reading in patients with dry eye. The British journal of ophthalmology. 2017 Apr ; 101 (4): 481-486. doi:10.1136/bjophthalmol-2015-308237.

Utine CA. Keratoprotez. MN Oftalmoloji. 2017 ; 24 (1): 115-124.

Zhu Y, Serpooshan V, Wu S, Demirci U, Chen P, Güven S. Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly. Methods Mol Biol. . 2017 Sep doi:10.1073/pnas.1708621114.

Contact

Guven Lab. on Therapeutic Bioengineering

Assoc.Prof. Sinan GUVEN
sinan.guven@ibg.edu.tr
+90 232 299 41 00 (5131)
+90 232 299 41 63