Guven Lab.

Therapeutic Bioengineering

OVERVIEW

Advances in stem cell biology and genetics with bioengineering science continuously lead breakthroughs and innovations in biotechnology. We harmonize fundamental sciences such as chemistry, biology, and physics with principles of engineering to generate innovative and effective therapeutic approaches, tissue mimicries and medical microdevices for medicine and pharmaceutical sciences. Our group focuses on bioengineering of novel native-like 3D cellular microenvironments, organ-on-a-chip platforms, and cell therapy approaches and integrates them for human health.

RESEARCH INTERESTS

Tissue and cellular microenvironment engineering: An understanding of how stem cells respond to physical and chemical stimulations from their extracellular environment is essential for utilizing the therapeutic potential of stem cells. We focus on developing novel biomaterials to construct cellular niches that can provide control over the stem cell differentiation and functionalization. We build tissues for regenerative medicine and pharmaceutical applications by designing and defining the cell-material and cell-cell interactions. Utilizing the bioengineering principles we develop bioreactor systems, tissue fabrication and assembly techniques for bottom-up and top-down tissue engineering.

Bio-mimicry organ-on-a-chip platform technologies for biomedical research: Organs-on-chips technologies provide a great platform for the investigation of fundamental mechanisms of organ physiology and disease. Such tools enable control over the physical and chemical factors of an in vitro organ models. Our group focuses on designing and engineering BioMEMS for basic research on cell physiology, in vitro disease models (e.g., neurodegenerative diseases, rare diseases, and cancer), diagnostics and pharmaceutical research.

Personalized and cellular therapies: Cell-based therapies provide unique and personalized approaches in current clinical treatments. Autologous and allogenic adult mesenchymal stem cells from bone marrow, adipose tissue, Wharton jelly and dental pulp, as well as limbal stem cells, and specialized cells (e.g., chondrocytes, hepatocytes) have been demonstrated to be effective in clinics. We develop novel therapeutic methods from bench to bedside by utilizing of cell-based therapies and intraoperative approaches in clinics.

RESEARCH HIGHLIGHTS

Biological organizations are highly hierarchical in architecture from the molecular to the macroscopic level. Such complexity in tissues is formed by building units that regulate and provide the system function. To mimic and bioengineer functional tissues, we need biomanufacturing tools that can generate and manipulate multi-scale building blocks. Bottom-up tissue engineering approaches focus on creating tissues by assembling heterogeneous building units such as biomacromolecules, cells, and cell-loaded microscale hydrogels in a multiscale manner. In our recent works, we demonstrated different cell and tissue assembly approaches utilizing acoustic, magnetic, micro robotic and bioprinting technologies to generate model platforms and tissues. Bioengineering approaches are essential for current therapeutic and diagnostic applications in medicine. Together with advances in genetics and cell biology, bioengineering tools provide key elements for novel and innovative medical technologies for human health.

 
Sinan Güven Şekil 2
Engineering platforms and systems for biofabrication of 3D tissues and microenvironments.
 Sinan Güven Şekil
Representative bioengineered tissue constructs A. Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue (Guven et al. 2011), B. In vitro endothelial capillaries from stromal vascular fraction (Guven et al. 2011), C. Enhanced engraftment and functionality of bio-engineered autologous dermo-epidermal skin grafts pre-vascularized with adipose-derived cells (Klar et al. 2014), D. Direct neural differentiation of mouse embryonic stem cells in 3D microenvironments, E. Biotunable acoustic node assembly of hepatic organoids (Chen et al. 2015), F. Guided and magnetic self-assembly of tunable magnetoceptive cardiac microtissues (Tasoglu et al. 2014).