Wingender Lab. on Mucosal Immunology


At the mucosal surfaces of the airways, intestine, and urogenital tract, the separation of our body from the environment is at times only one cell wide. These surfaces are colonized by a diverse mixture of microbial organisms, which under normal conditions, evolved to be beneficial. However, the mucosal surfaces are also a common entry point for pathogens and toxins. Therefore, the mucosal immune system has to fulfill two often-conflicting functions: to protect the host from harmful invaders, like pathogen; and support the function of the mucosa and to ensure the peaceful cooperation with the commensal microbiota. When this balance is disturbed, diseases are usually the consequence.


Our laboratory wants to better understand how the various immune cells in and around the mucosa cooperate to maintain its function and health. A particular focus is placed on innate-like T cells, iNKT and MAIT cells, which are innate memory T cells with several unique features (Wingender & Kronenberg 2020, Hapil & Wingender 2018). In our translational approach, we combine work with clinical samples, advanced in vitro models, and in vivo mouse models, to develop novel diagnostic and therapeutic applications.


1) The role of innate-like T cells in asthma and COPD: iNKT cells play a potent, but surprisingly dichotomous role in lung inflammation. During infections, their Th1-response is protective, whereas during allergic responses, their Th2-response is deleterious. We previously demonstrated the role of iNKT and MAIT cells in allergic asthma (Chandra et al. 2018, Wingender et al. 2011). We currently study three distinct forms of airway inflammation, (i) eosinophilic and (ii) neutrophilic asthma, as well as (iii) COPD (chronic obstructive pulmonary disease), to understand how iNKT and MAIT cell responses in the lung are regulated.

2) Impact of the intestinal microbiota on the adipose tissue: We previously showed that iNKT cells are impacted by the intestinal microbiota (Wingender et al. 2012, Wingender 2016) and that antigenic challenge of iNKT cells leads to long-lasting changes in the adipose tissue (Sag et al. 2014). We currently investigate how the adipose tissue is affected by translocation of intestinal bacteria into the peritoneum.

Other ongoing projects

· Death receptors: We investigate the role of TRAIL and DR4/DR5 interaction for the anti-tumor response of iNKT cells, in particular in the context of MM (multiple myeloma) and AML (acute myelogenous leukemia) (Sag et al. 2019).

· iNKT cell subsets: Similar to conventional CD4+ T cells, iNKT cells can be divided into several functional subsets. We previously discovered a novel subset of iNKT cells with potent regulatory functions, called NKT10 cells due to their production of IL-10 (Sag et al. 2014, Wingender et al. 2015a, 2015b). We are studying the in vivo regulation of these NKT10 cells in more detail and utilize preclinical studies to explore their therapeutic potential. Furthermore, ongoing projects investigate development and function of NKT2 and NKTFH cells.

· Rare diseases: IBG recently was awarded an ERA Chair project to establish itself as a Center of Excellence for Rare Disease research. In the context of this project, we currently launching a new line of research, focusing on rare diseases of the airways and of the immune system.

Group Members

Wingender Lab. on Mucosal Immunology

Research Group Leader

+90 232 299 41 00 (5191)
+9 0 (232) 299 41 69

Yonca ÜNLÜER Research Technician

Doğay BELEN Undergraduate Student

Aylin YAŞAR MSc Student

Zeynep Özge AYYILDIZ PhD Student

Yavuz MERCAN PhD Student

Former Members

Fatma Zehra HAPİL Research Group Member

Başak GÜNDÜZ MSc Student

Zeynep TANYOLAÇ MSc Student

Yusuf Cem ESKİOCAK Researcher

Sezgin BAL MSc Student

Müge ÖZKAN PhD Student

Selected Publications

Özkan M, Eskiocak YC, Wingender G. Macrophage and dendritic cell subset composition can distinguish endotypes in adjuvant-induced asthma mouse models.. PloS one. 2021 June ; 16 (6) : e0250533. doi:10.1371/journal.pone.0250533. Download

Özkan M, Eskiocak YC, Wingender G. The IL-10GFP (VeRT-X) mouse strain is not suitable for the detection of IL-10 production by granulocytes during lung inflammation.. PloS one. 2021 May ; 16 (5) : e0247895. doi:10.1371/journal.pone.0247895. Download

Sag D, Ayyildiz ZO, Gunalp S, Wingender G. The Role of TRAIL/DRs in the Modulation of Immune Cells and Responses.. Cancers. 2019 September ; 11 (10) : 1469. doi:10.3390/cancers11101469. Download

Hapil FZ, Wingender G. The interaction between invariant Natural Killer T cells and the mucosal microbiota. Immunology. 2018 October ; 155 (2) : 164-175. doi:10.1111/imm.12958. Download

Chandra S, Wingender G, Greenbaum JA, Khurana A, Gholami AM, Ganesan AP, Rosenbach M, Jaffee K, Gern JE, Wood R, O'Connor G, Sandel M, Kattan M, Bacharier L, Togias A, Horner AA, Kronenberg M. Development of asthma in inner-city children: Possible roles of MAIT cells and variation in the home environment. Journal of immunology (Baltimore, Md. : 1950). 2018 March ; 200 (6) : 1995-2003. doi:10.4049/jimmunol.1701525. Download

Duygu Sag, Müge Özkan, Mitchell Kronenberg & Gerhard Wingender. Improved Detection of Cytokines Produced by Invariant NKT Cells. Sci Rep. 2017 November ; 7 (1) : 16607. doi:10.1038/s41598-017-16832-1. Download

Wingender, Gerhard. From the Deep Sea to Everywhere: Environmental Antigens for iNKT Cells. ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS. 2016 August ; 64 (4) : 291-298. doi:10.1007/s00005-015-0381-7. Download

Wingender G, Sag D, Kronenberg M. NKT10 cells: a novel iNKT cell subset. Oncotarget. 2015 September ; 6 (29) : 26552-26553. doi:10.18632/oncotarget.5270. Download

Wingender, Gerhard; Kronenberg, Mitchell. OMIP-030: Characterization of Human T Cell Subsets via Surface Markers. CYTOMETRY PART A. 2015 December ; 87A (12) : 1067-1069. doi:10.1002/cyto.a.22788. Download

Wingender, Gerhard; Birkholz, Alysia M.; Sag, Duygu; Farber, Elisa; Chitale, Sampada; Howell, Amy R.; Kronenberg, Mitchell. Selective Conditions Are Required for the Induction of Invariant NKT Cell Hyporesponsiveness by Antigenic Stimulation. JOURNAL OF IMMUNOLOGY. 2015 October ; 195 (8) : 3838-3848. doi:10.4049/jimmunol.1500203. Download

Sag D, Krause P, Hedrick CC, Kronenberg M, Wingender G. IL-10-producing NKT10 cells are a distinct regulatory invariant NKT cell subset.. The Journal of clinical investigation. 2014 September ; 124 (9) : 3725-40. doi:10.1172/JCI72308. Download

Wingender G, Stepniak D, Krebs P, Lin L, McBride S, Wei B, Braun J, Mazmanian SK, Kronenberg M. Intestinal microbes affect phenotypes and functions of invariant natural killer T cells in mice.. Gastroenterology. 2012 August ; 143 (2) : 418-28. doi:10.1053/j.gastro.2012.04.017. Download

Wingender G, Rogers P, Batzer G, Lee MS, Bai D, Pei B, Khurana A, Kronenberg M, Horner AA. Invariant NKT cells are required for airway inflammation induced by environmental antigens.. The Journal of experimental medicine. 2011 June ; 208 (6) : 1151-62. doi:10.1084/jem.20102229. Download

Total : 13

Selected Book Chapters

Chapter 7. The role of invariant Natural Killer T cells in autoimmune diseases (2020). The Autoimmune Diseases (6th edition). Elsevier.

Chapter 8. The role of invariant Natural Killer T cells in autoimmune diseases (2014). In: The Autoimmune Diseases (5th edition). Elsevier.

Total : 2


The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Characteristics And Plasticity of INKT Cell Subsets In Mouse And Human, Finished

European Molecular Biology Organization - EMBO - RD : Regulation of Airway Inflammations By Invariant Natural Killer T (INKT) Cells During Allergen Induced Hypersensitivity And Infection, Ongoing

The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Functional characteristics and transcription factor usage of mouse NKT10 cells, Finished

The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Cell-cell interactions of iNKT cells during airway hypersensitivity responses, Finished

European Union - RD : Death Receptor Signalling in Tumour Immune Editing, Ongoing

DEÜ BAP - Dokuz Eylul University Scientific Research Projects Coordination Unit - RD : Implementing technologies and methodologies for the investigation of invariant Natural Killer T (iNKT) cells, Finished

DEÜ BAP - Dokuz Eylul University Scientific Research Projects Coordination Unit - RD : İnvariyant Doğal Öldürücü T (iNKT) hücrelerinin alerjen kaynaklı akciğere infiltrasyonu / Allergen induced lung infiltration of invariant Natural Killer (iNKT) cells, Finished

The Scientific and Technological Research Council of Turkey - TUBITAK - RD : The role of TRAIL mediated signals on the proliferation and function of human iNKT cells, Ongoing

DEÜ BAP - Dokuz Eylul University Scientific Research Projects Coordination Unit - RD : The role of invariant natural killer T (iNKT) cells in neutrophilic, Th1-driven, asthma, Ongoing

Open Positions

Our laboratory always considers qualified applications of post-doctoral fellows and of prospective graduate students (Ph.D.) with a background in immunology or bioinformatics. As of July 2022, candidates with a bioinformatic background will be favoured.

If you are interested in advancing immunology in a top-notch, collaborative environment, then please contact Gerhard Wingender directly ( with your CV and a detailed letter of motivation.

Furthermore, if qualified prospective candidates fulfil the mobility requirements of MSCA or EMBO, we will actively work with them and guide them through the complete application process. Keep in mind that applicants to MSCA fellowships planning to work in Turkey will have the benefit to skip the first selection round and enter the second one directly (this is new under Horizon Europe).


  • Above-Threshold Award by TUBITAK, 2017
  • Installation Grant by EMBO, 2015

Academic Memberships

  • American Association of Immunologists (AAI), 2010
  • European Association for Cancer Research (EACR), 2017
  • German Society for Immunology (DGfI), 2001
  • Society for Mucosal Immunology (SMI), 2017
  • Turkish Association for Molecular Cancer Research (MOKAD), 2017
  • Turkish Society of Immunology (TSI), 2016
  • International Society for Advancement of Cytometry (ISAC), 2020


Wingender Lab. on Mucosal Immunology

Research Group Leader

+90 232 299 41 00 (5191)
+9 0 (232) 299 41 69