IBG Gene Targeting and Transgenic Models Platform has recently established its research laboratories and state of the art technological infrastructure. Our platform will provide services and collaborative support to internal and external researchers working in basic and translational biomedical sciences, whose work involve or require the generation of genetically-engineered mouse models.
In addition to the services we offer below, our lab is interested in investigating rare genetic disorders utilizing transgenic mouse models.
Generation of knockout and knockin mouse models:
Our research platform invites researchers to collaborate with us for the generation of knockout and knockin mouse models for their favourite genes. We offer collaborations starting from initial planning and vector design stage to the final establishment of a genetically modified mouse colony.
Mouse embryo cryopreservation and rederivation:
The technological infrastructure for embryo cryopreservation and rederivation has been recently completed. After establishment of the relevant protocols, we will provide services to internal and external users who wish to store their transgenic mouse strains long term.
Our platform has recently created a genetically modified (transgenic) mouse model for the first time in our laboratory. We have edited the Tyr gene in C57BL/6J mice by CRISPR genome engineering technology. The knockin mutation we introduced, causes loss of function in the tyrosinase enzyme, resulting in albino skin color.
C57BL/6J is the most widely used mouse strain in biomedical research. Albino C57BL/6J mice are the preferred model for bioluminescence imaging studies due to their lack of skin pigment melanin. Additionally, their embryos are commonly used for microinjection of ES cells derived from BL6 strains. This newly created strain will be a valuable resource for IBG Vivarium and our transgenic platform.
We are currently in the process of creating several knockin mouse lines to model rare genetic disease in vivo.
Our ongoing work is funded by the TUBITAK 1005 program.
28 September 2021
The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Transgenik fare modellerinin yerel üretimi için yöntem geliştirilmesi, Ongoing