Gene Targeting and Transgenic Models Platform

OVERVIEW

IBG Gene Targeting and Transgenic Models Platform has recently established its research laboratories and state of the art technological infrastructure. Our platform will provide services and collaborative support to internal and external researchers working in basic and translational biomedical sciences, whose work involve or require the generation of genetically-engineered mouse models.

In addition to the services we offer below, our lab is interested in investigating rare genetic disorders utilizing transgenic mouse models.

COLLABORATIVE SUPPORT AND SERVICES

Generation of knockout and knockin mouse models:

Our research platform invites researchers to collaborate with us for the generation of knockout and knockin mouse models for their favourite genes. We offer collaborations starting from initial planning and vector design stage to the final establishment of a genetically modified mouse colony.

We have, quite recently, created our first knockin mouse model for a new rare genetic disorder (see News tab). We are currently developing several transgenic disease models. We are ready to collaborate with interested partners who need to generate novel genetically modified mouse models.

Mouse embryo cryopreservation and rederivation:

The technological infrastructure for embryo cryopreservation and rederivation has been recently completed. After establishment of the relevant protocols, we will provide services to internal and external users who wish to store their transgenic mouse strains long term.

SERVICES - Currently provided

Services we currently provide are listed below. Please contact us via e-mail for further details and conditions of the services.

1. Knockout (indel)

2. Knockout (deletion)

3. Knockin (point mutations)

4. Knockin (epitope tags)

5. ESC gene targeting

SERVICES - In development

The services listed below are within our technical epertise,  although we do not routinely apply these protocols.

1. Sperm cryopreservation

2. Embryo cryopreservation

3. IVF (in vitro fertilization)

4. Embryo transfer and mouse strain rederivation

5. ESC microinjection

6. PNI (pronuclear injection)

Personnel

Gene Targeting and Transgenic Models Platform

Research Group Leader

Kasım DİRİL
kasim.diril@ibg.edu.tr
+90 232 299 41 00 (5091)
+9 0 232 299 41 59

Kerem ESMEN R&D Personnel  kerem.esmen@ibg.edu.tr


Seval KILIÇ PhD Student  seval.kilic@ibg.edu.tr


Aslı BABAYAKALI MSc Student  asli.babayakali@ibg.edu.tr


Gizem ÖZTÜRK PhD Student  gizem.ozturk@ibg.edu.tr

0 (232) 299 _ _

Pelin Dide BEŞER Undergraduate Student  pelindide.beser@ibg.edu.tr


Cengizhan BİLGİN Undergraduate Student  cengizhan.bilgin@ibg.edu.tr


Former Personnel

Liubovi SOPCO Undergraduate Student  liubovi.sopco@msfr.ibg.edu.tr


Mehmet ERGÜVEN MSc Student  mehmet.erguven@msfr.ibg.edu.tr


Mehmet ERGÜVEN Visiting Researcher  mehmet.erguven@msfr.ibg.edu.tr


Ferzane VALİOĞLU Post-Doc Researcher  ferzane.valioglu@ibg.edu.tr


Gülin KÖKLÜ Undergraduate Student  gulin.koklu@ibg.edu.tr


Gizem ÖZTÜRK MSc Student  gizem.ozturk@ibg.edu.tr

0 (232) 299 _ _

News

IFNAR1 KO mice

IBG Transgenic Platform has developed the IFNAR1 knockout mouse model. The IFNAR1 gene codes for the type-I interferon-α/β receptor, which activates JAK-STAT signaling pathways upon binding type-I IFN.  JAK-STAT signaling is essential for processes including growth regulation, survival, differentiation, pathogen resistance and antiviral immune responses. IFNAR1 knockout mice do not display type-I IFN receptor function which causes reduced immune response and an increased susceptibility to viral infection. IFNAR1 knockout mice are widely used in research on antiviral immune responses, and interferon stimulation and JAK-STAT signaling.


Expanding our transgenic models repertoire

Recently, we have added two knockin and one knockout mouse models to our platform's repertoire. The total number of home-made transgenic models is now five.

One of the knockin strains we engineered, will be used to study a newly defined neurodevelopmental rare genetic disorder.

The other mouse models are for the investigation of gene functions in vivo.

At this stage, we can confidently offer knockin or knockout models as a service to academia.


Our first rare genetic disease model was created

We have recently created our first transgenic model for a novel rare genetic disease. The mutation in the patients causes  truncation of the cytoplasmic tail domain of a multi-pass transmembrane protein. To mimic the mutation in mice, we have engineered the mouse genomic locus and introduced a STOP codon into the relevant coding exon. This results in truncation of the protein product- a transmembrane protein with a shorter cytosolic domain. These mice will serve as an in vivo model for the characterization of the disease.

 

12 December 2021


Albino C57BL/6J transgenic mice

Our platform has recently created a genetically modified (transgenic) mouse model for the first time in our laboratory. We have edited the Tyr gene in C57BL/6J mice by CRISPR genome engineering technology. The knockin mutation we introduced, causes loss of function in the tyrosinase enzyme, resulting in albino skin color.

C57BL/6J is the most widely used mouse strain in biomedical research. Albino C57BL/6J mice are the preferred model for bioluminescence imaging studies due to their lack of skin pigment melanin. Additionally, their embryos are commonly used for microinjection of ES cells derived from BL6 strains. This newly created strain will be a valuable resource for IBG Vivarium and our transgenic platform.

We are currently in the process of creating several knockin mouse lines to model rare genetic disease in vivo.

Our ongoing work is funded by the TUBITAK 1005 program.

28 September 2021


Selected Publications

Erguven M, Kilic S, Karaca E, Diril MK.. Genetic complementation screening and molecular docking give new insight on phosphorylation-dependent Mastl kinase activation. Journal of Biomolecular Structure and Dynamics. 2022 October . doi:10.1080/07391102.2022.2131627. Download

Erguven M, Karakulak T, Diril MK, Karaca E. How Far Are We from the Rapid Prediction of Drug Resistance Arising Due to Kinase Mutations?. ACS omega. 2021 January ; 6 (2) : 1254-1265. doi:10.1021/acsomega.0c04672. Download

Szmyd R, Niska-Blakie J, Diril MK, Renck Nunes P, Tzelepis K, Lacroix A, van Hul N, Deng LW, Matos J, Dreesen O, Bisteau X, Kaldis P.. Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality. Oncogene. 2019 February ; 38 (7) : 998-1018. doi:10.1038/s41388-018-0464-0. Download

Total : 3

Projects

The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Analysis of mitotic functions of ENSA and Arpp19 proteins, Finished

DEÜ BAP - Dokuz Eylul University Scientific Research Projects Coordination Unit - RD : Mastl kinazın mitotik fonksiyonlarının kimyasal genetik yöntemlerle analizi, Finished

The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Transgenik fare modellerinin yerel üretimi için yöntem geliştirilmesi, Ongoing

The Scientific and Technological Research Council of Turkey - TUBITAK - RD : Generation and phenotypic characterization of a transgenic mouse model for a neurodevelopmental rare genetic disorder, Ongoing

Open Positions

Postdoctoral researcher

We are looking for a postdoctoral researcher to join our research team working on rare genetic disorders.

The candidate is expected to apply to the TÜBİTAK BİDEB 2218 program for financial support.

The project proposal should involve creation of a transgenic mouse model for a novel or known rare genetic disorder with subsequent phenotyping analyses. Help and guidance will be provided for selecting a suitable project topic and writing of the proposal.

Candidates should  i) have a very good command of English, ii) able and willing to work with mouse models, and iii) be experienced in basic molecular biology techniques (PCR, cloning, restriction analysis etc.).

Please see the details in the link provided below (in Turkish).

https://www.tubitak.gov.tr/tr/burslar/doktora-sonrasi/arastirma-burs-programlari/icerik-2218-yurt-ici-doktora-sonrasi-arastirma-burs-programi

Awards

  • Young Investigator (GEBİP) Awards by Turkish Academy of Sciences (TÜBA), 2017
  • Young Scientist (BAGEP) Award by Science Academy, 2016
  • 2232 Yurda Dönüş Araştırmacı Dolaşım Programı by TUBITAK, 2015

Contact

Gene Targeting and Transgenic Models Platform

Research Group Leader

Kasım DİRİL
kasim.diril@ibg.edu.tr
+90 232 299 41 00 (5091)
+9 0 232 299 41 59