Flow Cytometry And Cell Sorting Unit

Flow cytometry is an advanced laser-based analytical technology that enables simultaneous, multiparametric analysis of the physical and/or chemical properties of cells at the single-cell level. Cells in suspension are hydrodynamically aligned to pass individually through the flow system and are interrogated by lasers at different wavelengths. Signals related to cellular parameters such as cell size, granularity, light scatter characteristics, and relative fluorescence intensity are filtered through optical systems and detected by photodetectors. The acquired data are converted into digital electronic signals and recorded for advanced analysis using dedicated software platforms.

With our specialized technical staff and years of experience in the field of flow cytometry, we provide reliable, high-quality services to research projects in accordance with international standards.

Instrumentation

BD FACSAria III – Cell Sorting
BD LSRFortessa – Cell Analysis
BD FACSCanto II – Cell Analysis

Analytical Services Provided

Multicolor flow cytometric analyses
Immunophenotyping of mammalian live-cell samples
Viability and protein expression analyses in non-pathogenic bacteria and yeast
Analysis of intracellular signaling pathways
Assessment of cellular activation status and oxidative burst
Cell viability, apoptosis, and proliferation analyses
Mitochondrial membrane potential measurements
DNA content analysis, polyploidy detection, and cell cycle profiling
Gene expression and gene transfer assessments (e.g., transfection efficiency)
Intracellular calcium flux measurements
Simultaneous analysis of surface and intracellular markers (e.g., intracellular cytokine levels)

Cell Sorting Services

Bulk cell sorting
Simultaneous sorting of up to four distinct cell populations
Single-Cell Sorting: Individual cell deposition into 96- and 384-well plate formats
High-purity and high-precision isolation of target cell populations
Sorting with multiple nozzle size options (70 µm, 85 µm, 100 µm, and 130 µm) according to cell size

Publications Related to Flow Cytometry

1.Yılmaz A, Özbilgiç R, Polatlı E, Erbay İH, Sağ D, Güven S.: Macrophage Polarization Profiling in Dynamic Culture System. Cel. Mol. Bioeng. (2025). https://doi.org/10.1007/s12195-025-00863-0

2. Alperen Yılmaz, Resul Özbilgiç, Elifsu Polatlı, İbrahim Halilullah Erbay, Duygu Sağ & Sinan Güven: Development of a mouse embryonic stem cell model for investigating the functions of the linker histone H1-4 https://doi.org/10.1002/2211-5463.13750

3. Eskiocak YC, Ayyildiz ZO, Gunlap S, Korkmaz A, Sag D, Wingender G: The Ca2+ concentration impacts the cytokine production of mouse and human lymphoid cells and the polarization of human macrophages in vitro2023 https://pubmed.ncbi.nlm.nih.gov/36827279/

4. Ozkan M, Eskiocak YC, Wingender G: Macrophage and dendritic cell subset composition can distinguish endotypes in adjuvant-induced asthma mouse models https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250533

5. Ozkan M, Eskiocak YC, Wingender G: The IL-10GFP (VeRT-X) mouse strain is not suitable for the detection of IL-10 production by granulocytes during lung inflammation. https://doi.org/10.1371/journal.pone.0247895

6. Sag D, Ozkan M, Kronenberg M and Wingender G: Improved Detection of Cytokines Produced by Invariant NKT Cells https://www.nature.com/articles/s41598-017-16832-1

7.Soheil Akbari, GülbenGürhan Sevinç, Nevin Ersoy, Onur Basak, Kubra Kaplan, Kenan Sevinç,Erkin Ozel, Berke Sengun, Eray Enustun, Burcu Ozcimen, Alper Bagriyanik , Nur Arslan , Tamer Tevfik Önder , Esra Erdal: Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for DiseaseModeling https://doi.org/10.1016/j.stemcr.2019.08.007

8. Pelin Saglam-Metiner, Utku Devamoglu, Yagmur Filiz, Soheil Akbari, Goze Beceren, Bakiye Goker, Burcu Yaldiz, Sena Yanasik, Cigir Biray Avci, Esra Erdal & Ozlem Yesil-Celiktas: Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids https://.nature.com/articles/s42003-023-04547-1.pdf?

9. Zeynep Firtina Karagonlar, Soheil Akbari, Mustafa Karabicici, Eren Sahin, Sanem Tercan Avci, Nevin Ersoy, Kıvılcım Eren Ates, Tugsan Balli, Bilge Karacicek, Kubra Nur Kaplan, Canan Celiker, Nese Atabey, Esra Erdal: A Novel Function for KLF4 in Modulating the De-Differentiation of EpCAM−/CD133− nonStem Cells into EpCAM+/CD133+ Liver Cancer Stem Cells in HCC Cell Line HuH7 https://doi.org/10.3390/cells9051198

10. Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal: Modeling hepatic fibrosis in TP53 knockout iPSC-derived human liver organoids https://pubmed.ncbi.nlm.nih.gov/41065540/

11. Pelin Saglam-Metiner, Elif Duran, Leila Sabour-Takanlou, Cigir Biray-Avci, Ozlem Yesil-Celiktas: Differentiation of Neurons, Astrocytes, Oligodendrocytes and Microglia From Human Induced Pluripotent Stem Cells to Form Neural Tissue-On-Chip: A Neuroinflammation Model to Evaluate the Therapeutic Potential of Extracellular Vesicles Derived from Mesenchymal Stem Cells https://link.springer.com/article/10.1007/s12015-023-10645-8#Sec24

12. Pelin Saglam-Metiner, Utku Devamoglu, Yagmur Filiz, Soheil Akbari, Goze Beceren, Bakiye Goker, Burcu Yaldiz, Sena Yanasik, Cigir Biray Avci, Esra Erdal & Ozlem Yesil-Celiktas: Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids https://www.nature.com/articles/s42003-023-04547-1

13. Unuvar Purcu, D., Korkmaz, A., Gunalp, S., Helvaci, D. G., Erdal, Y., Dogan, Y., Suner, A., Wingender, G., Sag, D. (2022). Effect of stimulation time on theexpression of human macrophage polarization markers. https://doi.org/10.1371/journal.pone.0265196

14. Gunalp, S., Helvaci, D. G., Oner, A., Bursalı, A., Conforte, A., Güner, H., Karakülah, G., Szegezdi, E., & Sag, D. (2023). TRAIL promotes the polarization of human macrophages toward a proinflammatory M1 phenotype and is associated with increased survival in cancer patients with high tumor macrophage content. Frontiers in Immunology, 14, 1209249. https://doi.org/10.3389/fimmu.2023.1209249

15. Korkmaz, A., Unuvar, D., Gunalp, S., Helvaci, D. G., & Sag, D.: Kabasura kudineer choornam, a medicinal polyherbal formulation, modulates human macrophage polarization and phagocytic function. Turkish Journal of Biology, 49(4), 348–366. https://doi.org/10.55730/1300-0152.2752

16. Eda Argitekin Esra Ersoz-GulsevenGulcin Cakan-AkdoganYasar Akdogan: Dopamine-Conjugated Bovine Serum Albumin Nanoparticles Containing pH-Responsive Catechol-V(III) Coordination for In Vitro and In Vivo Drug Delivery https://pubs.acs.org/doi/10.1021/acs.biomac.3c00363?hl=tr-TR

17. Aysegul Kaymak Ozdemir and Mahinur Basci: Elucidating the role of ZRF1 in monocyte-to-macrophage differentiation, cell proliferation and cell cycle in THP-1 cells https://www.degruyterbrill.com/document/doi/10.1515/tjb-2024-

Researchers / Senior Researchers

Hatice Asena Şanlı	Researcher
Zeynep Karakan Karakaş	Senior Researcher

Unit Coordinator:

Reservation